Generator

Part:BBa_K1316013:Design

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-09-29)


pRhamnose regulating csgB + J23110 constitutive Anderson promoter regulating csgA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 516
    Illegal NheI site found at 551
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Many more CsgA protein subunits are required compared to CsgB in curli formation.


Source

csgA and csgB were PCRed out of the K540000 Lyon 2011 iGEM part.

References